Background: 99mTc-sestamibimyocardial perfusion imaging (MIBI) washout is associated withmyocardial mitochondrial damage in patients with a successful percutaneous coronary intervention (PCI) following acutemyocardial infarction (AMI) andmaypredict the functional improvement of the left ventricle in follow-ups. Objectives: This study aimed to investigate the clinical efficacy of 99mTc-MIBI washout in patients with AMI by measuring the mean defect area based on 99mTc-MIBI myocardial perfusion-single photon emission computed tomography (MP-SPECT) rest imaging in early and delayed phases and comparing it with the defect area based on 99mTc-MIBI MP-SPECT adenosine stress imaging based on a Two-day rest/stress Protocol. Patients and Methods: This study was conducted on 29 consecutive patients with AMI (23 males and 6 females,mean age, 71, 8. 4 years), who underwent MP-SPECT using a standard Two-day rest/stress Protocol. The rest 99mTc-MIBI MP-SPECT images were acquired in the early phase at one hour after the injection of 99mTc-MIBI and in the delayed phase at three hours after the early phase. The total perfusion deficit (TPD) score for SPECT was measured to compare the defect area between the rest-early phase, rest-delayed phase, and post-stress imaging conditions. Results: Based on the results, the post-stress TPD score was significantly lower than the rest-delayed phase score (TPD: 22. 2% , 14. 3% vs. 27. 8% , 14. 0%,P < 0. 001). Also, the rest-early phase score was significantly lower than the rest-delayed phase score (TPD: 21. 5% ,14. 9% vs. 27. 8% , 14. 0%,P < 0. 001). However, no significant difference was observed between the post-stress score and the rest-early phase score. Conclusion: The combination of rest-early phase, delayed phase, and post-stress 99mTc-MIBI imaging using a Two-day Protocol after AMI reperfusion was a clinically useful method, which could identify residual ischemia and predict the left ventricular function improvement in the chronic phase of disease while reducing the exposure dose.

Background: Brain perfusion SPECT imaging has been a useful way to evaluate cerebro‐vascular diseases and other neuro diseases. Two‐day Protocol brain perfusion SPECT is more accurate but not convenient for most patients. This is the reason why a one – day Protocol using SPECT/CT was designed.Materials and Methods: Both rest and acetazolamide was done by SPECT/CT system. A 2 minute ‐ dynamic acquisition was carried out immediately after injection of 99mTc ‐ HMPAO. Then, the 1st rest SPECT study was done for 30 min after 3 min post injection of 370 MBq (10 mCi) of 99mTc ‐ HMPAO. Immediately after the 1st SPECT, 1g of acetazolamide was given intravenously. Ten minutes later, 740 MBq (20 mCi) of 99mTc – HMPAO was additionally injected. Three minutes later the 2nd SPECT study was carried out for 30 min. To measure regional cerebral blood flow (rCBF) non‐invasively, the Patlak plot analysis was applied to time–activity data on radionuclide angiography. Then reconstructed images from 1st SPECT were converted to 1st rCBF map. Base on the increased count ratio in cerebellum between decay corrected 1st and 2nd SPECT images, acetazolamide increasing ratio was calculated, and then 2nd rCBF map was generated. Region of interest (ROI) in 1st and 2nd images were set, then %increase values were calculated.Results: Thirty‐three patients were performed. The rest cortices showed rCBF values by 49.3±3.3 ml/100g brain/min, acetazolamide enhanced cortices show rCBF values by 53.8±4.0 ml/100g brain/min and % increase value by 8.9±3.7 on average.Conclusion: This method of imaging would be significant for evaluating cerebral perfusion reserve in the routine cerebro‐vascular disease for a comfortable method to patient because they do not have to come to hospital in 2 days for scanning.

Background: Pregnancy is a successful transplantation. The factors evading rejection of the fetus are poorly understood. Recently an interest has grown, in HLA-G molecules as one of these factors. Since these antigens are mainly expressed on the surface of cytotro-phoblasts that are in direct contact with maternal tissues, it has been suggested that these molecules may have a role in induction of immune tolerance in mothers. Objective: to find the association of soluble HLA-G (sHLA-G) and the success of pregnancy with intracyto-plasmic sperm injection (ICSI) procedure. Methods: In this study, the supernatant of 287 individually cultured embryos corresponding to 96 women under ICSI procedure were as-sayed for soluble HLA-G1 and G5 by a sandwich ELISA. Results: Clinical pregnancy suc-cessfully occurred in 30 of candidates. No differences in clinical parameters (age, infertility duration, stimulation regimen) were observed between pregnant and nonpregnant women under ICSI procedure. None of the embryo supernatants in either group showed any detect-able sHLA-G molecules. Conclusion: Our results showed that detectable level of sHLA-G is not produced by day 2 embryos and such a measurement may not provide reliable infor-mation for embryo selection and estimation of pregnancy success.